dna tethers Search Results


dna tethers  (Bayou Biolabs)
90
Bayou Biolabs dna tethers
Dna Tethers, supplied by Bayou Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/pmc04047793-80-7-60?v=Bayou+Biolabs
Average 90 stars, based on 1 article reviews
dna tethers - by Bioz Stars, 2026-08
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analyte-induced tethering of micron-sized beads by long dna tethers  (Scanogen Inc)
90
Scanogen Inc analyte-induced tethering of micron-sized beads by long dna tethers
Analyte Induced Tethering Of Micron Sized Beads By Long Dna Tethers, supplied by Scanogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/pmc10037498-131-9-4?v=Scanogen+Inc
Average 90 stars, based on 1 article reviews
analyte-induced tethering of micron-sized beads by long dna tethers - by Bioz Stars, 2026-08
90/100 stars
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dna tethering kit  (Lumicks B.V)
90
Lumicks B.V dna tethering kit
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
Dna Tethering Kit, supplied by Lumicks B.V, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/pmc11512004-241-2-2?v=Lumicks+B.V
Average 90 stars, based on 1 article reviews
dna tethering kit - by Bioz Stars, 2026-08
90/100 stars
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double-stranded dna tethers labeled with ht ligand  (Promega)
90
Promega double-stranded dna tethers labeled with ht ligand
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
Double Stranded Dna Tethers Labeled With Ht Ligand, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/pmc04410804-154-5-7?v=Promega
Average 90 stars, based on 1 article reviews
double-stranded dna tethers labeled with ht ligand - by Bioz Stars, 2026-08
90/100 stars
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dna tether  (Verlag GmbH)
90
Verlag GmbH dna tether
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
Dna Tether, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/pm20521266-53-17-15?v=Verlag+GmbH
Average 90 stars, based on 1 article reviews
dna tether - by Bioz Stars, 2026-08
90/100 stars
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g-quadruplex dna–berberine encapsulated by a planar side arm–tethered β-cyclodextrin  (Biomol GmbH)
90
Biomol GmbH g-quadruplex dna–berberine encapsulated by a planar side arm–tethered β-cyclodextrin
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
G Quadruplex Dna–Berberine Encapsulated By A Planar Side Arm–Tethered β Cyclodextrin, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/10__1016_slash_j__molstruc__2022__132838-276-6-16?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
g-quadruplex dna–berberine encapsulated by a planar side arm–tethered β-cyclodextrin - by Bioz Stars, 2026-08
90/100 stars
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finite element analysis for tethered dna in a shear flow  (COMSOL Inc)
90
COMSOL Inc finite element analysis for tethered dna in a shear flow
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
Finite Element Analysis For Tethered Dna In A Shear Flow, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/10__1039_slash_c6ra08683g-89-8-10?v=COMSOL+Inc
Average 90 stars, based on 1 article reviews
finite element analysis for tethered dna in a shear flow - by Bioz Stars, 2026-08
90/100 stars
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aptamer-tethered dna nanotrains  (Verlag GmbH)
90
Verlag GmbH aptamer-tethered dna nanotrains
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
Aptamer Tethered Dna Nanotrains, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/pm25277749-102-40-22?v=Verlag+GmbH
Average 90 stars, based on 1 article reviews
aptamer-tethered dna nanotrains - by Bioz Stars, 2026-08
90/100 stars
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non-fusogenic dna–lipid mimic of tethering factors  (Verlag GmbH)
90
Verlag GmbH non-fusogenic dna–lipid mimic of tethering factors
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
Non Fusogenic Dna–Lipid Mimic Of Tethering Factors, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/10__1002_slash_anie__201584811-161-12-8?v=Verlag+GmbH
Average 90 stars, based on 1 article reviews
non-fusogenic dna–lipid mimic of tethering factors - by Bioz Stars, 2026-08
90/100 stars
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benzylidene of a dna-tethered phosphonium salt  (Verlag GmbH)
90
Verlag GmbH benzylidene of a dna-tethered phosphonium salt
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
Benzylidene Of A Dna Tethered Phosphonium Salt, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/pm22441837-50-28-6?v=Verlag+GmbH
Average 90 stars, based on 1 article reviews
benzylidene of a dna-tethered phosphonium salt - by Bioz Stars, 2026-08
90/100 stars
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acridine-tethered dna  (Makoto USA Inc)
90
Makoto USA Inc acridine-tethered dna
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
Acridine Tethered Dna, supplied by Makoto USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/10__1246_slash_cl__2000__1378-43-15-23?v=Makoto+USA+Inc
Average 90 stars, based on 1 article reviews
acridine-tethered dna - by Bioz Stars, 2026-08
90/100 stars
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dna-lipid tether system  (Rothmans Inc)
90
Rothmans Inc dna-lipid tether system
a Cartoon schematic showing how 5fC was incorporated into nick translated λ <t>DNA.</t> Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 <t>kb</t> <t>LUMICKS</t> handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.
Dna Lipid Tether System, supplied by Rothmans Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+tethers/pm35025403-115-28-24?v=Rothmans+Inc
Average 90 stars, based on 1 article reviews
dna-lipid tether system - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


a Cartoon schematic showing how 5fC was incorporated into nick translated λ DNA. Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 kb LUMICKS handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.

Journal: Nature Communications

Article Title: Thymine DNA glycosylase combines sliding, hopping, and nucleosome interactions to efficiently search for 5-formylcytosine

doi: 10.1038/s41467-024-53497-7

Figure Lengend Snippet: a Cartoon schematic showing how 5fC was incorporated into nick translated λ DNA. Created in BioRender. Schnable, B. (2023) BioRender.com/q21t266. b Cartoon schematic showing 28 base pair duplex DNA containing a single 5fC (orange) ligated into 6 kb LUMICKS handle kit, with handle 2 containing ATTO 488 (blue). Created in BioRender. Schnable, B. (2024) BioRender.com/z84c645. c A diagram depicting the order of reagents, which are under laminar flow, are captured in the flowcell. Created in BioRender. Schnable, B. (2023) BioRender.com/k78o196. d A cartoon depiction of the DNA substrate used for TDG binding, with 5fC sites shown in blue, and an example kymograph with TDG binding shown in red. Specific event indicated with gray asterisk and nonspecific event indicated with teal asterisk. e Cumulative Resident Time Distribution (CRTD) analysis fit to a two-phase decay of TDG binding DNA containing 5fC specifically ( n = 70) and nonspecifically ( n = 487). Data represents the mean ± SEM from four independent experiments. f A cartoon depiction of the unmodified DNA substrate with an example kymograph of TDG binding and moving. g CRTD analysis fit to a one-phase decay of TDG binding unmodified λ DNA ( n = 155). Data represents the mean ± SEM of the fit from three independent experiments. h An example kymograph of TDG binding to a single 5fC. i CRTD analysis fit to a two-phase decay of TDG binding to 5fC ( n = 28). Data represents the mean ± SEM of the fit from three independent experiments.

Article Snippet: Using the LUMICKS DNA tethering kit and protocol, a sequence of interest is ligated into two handles that are each 6.3 kb in length to generate a substrate that is 12.6 kb in total.

Techniques: Binding Assay